Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii

Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii.

The utility of recombinant Toxoplasma gondii surface antigen P22 for the detection of specific T. gondii antibodies in human sera was evaluated. Polymerase chain reaction was used to produce a 438-bp fragment of the P22 gene; the fragment corresponded to the amino acids predicted to be in the processed, native antigen. The fragment was subcloned into pGEX-2T and was expressed in Escherichia col...

Recombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii

Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Reco...

expression and purification of p43 toxoplasma gondii surface antigen

background : toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. congenital toxoplasmosis is more important during pregnancy for the first time. in this study we expressed and purified p43 toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. methods : the recombinant pgemex-1 contained toxoplasma p43 codi...

Expression and Purification of P43 Toxoplasma gondii Surface Antigen

BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. Congenital toxoplasmosis is more important during pregnancy for the first time. In this study we expressed and purified P43 Toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. METHODS The recombinant pGEMEX-1 contained Toxoplasma P43 coding...

High-level expression and evaluation of the antigenicity of a recombinant Toxoplasma gondii GRA2 Protein